Quantitative Proteome Analysis: Methods and Applications

Quantitative analysis of the Escherichia coli proteome

Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article.

Methods and Applications of Quantitative Linguistics

Preface The contributions to this volume are selected and peer-reviewed papers on the basis of talks which were delivered on the QUALICO 2012 was, as usual, open to original papers without any limitations as to thematic or methodological aspects. Therefore, a large variety of topics has been addressed and numerous fields of linguistics and related fields have been touched. A number of contribut...

Sequential Methods and Their Applications

Mass Spectrometry-based Methods of Proteome Analysis

1 Principles and Instrumentation 3 1.1 Proteome and Proteomics 3 1.2 Need for Large-scale Analyses of Gene Products at the Protein Level 3 1.3 General Problems in Proteome Analyses 4 1.4 MS Instrumentation 5 1.4.1 Ionization Methods 6 1.4.2 Mass Analyzers 8 1.4.3 MS/MS 9 1.5 Methods of Sample Fractionation 10 1.5.1 2D-electrophoretic Separation of Complex Protein Mixtures 11 1.5.2 Liquid-phase ...

Quantitative validation of different protein precipitation methods in proteome analysis of blood platelets.

For the preparation of proteins for proteome analysis, precipitation is frequently used to concentrate proteins and to remove interfering compounds. Various methods for protein precipitation are applied, which rely on different chemical principles. This study compares the changes in the protein composition of human blood platelet extracts after precipitation with ethanol (EtOH) or trichloroacet...